Taq DNA Polymerase:

It is an Ultra pure and highly thermostable recombinant Taq DNA Polymerase that has been isolated from recombinant E.coli. The same is supplied with 10X PCR Buffer and an extra 100 mM Magnesium sulphate solution for condition optimisation with divalent ion. The enzyme possesses an inherent 5' to 3' exonuclease activity but lacks the 3' to 5' exonuclease or "proof reading" activity. On PCR Taq DNA polymerase leaves an 'A' at 3' end due to terminal transferase activity which is ideal for cloning in T-tailed vectors. It is free of any contaminating nucleases and is suited for PCR amplification of DNA including applications like Diagnostics, genomics, RAPD, AP-PCR etc.

RECOMBINANT THERMOSTABLE
Taq DNA Polymerase (VARDA's brand 'VarTeq') High lights:

• Ultra pure recombinant Taq DNA Polymerase from Thermus aquaticus

• Highly thermostable Taq DNA Polymerase

• Capable of catalyzing polymerization at the high temperature required for stringent and specific DNA amplification as well as withstanding even higher temperature necessary to separate the two DNA strands.

• For short and long PCR amplification of DNA

• For applications involving general and specialized PCR including diagnostic PCR as well as in RAPD to detect variations in prokaryotes and eukaryotes

• On PCR Taq DNA polymerase leaves an ‘A’ at 3’ end due to terminal transferase activity which is ideal for cloning in T-tailed vectors

• Ideal for extreme PCR conditions

• With PCR buffer (10X) and extra 100 mM MgSO4 solution for condition optimization with divalent ion, for minimal PCR optimization for optimal amplification of DNA, minimizing smearing and virtually eliminating background artifacts

Source: The most extensively studied thermostable DNA polymerase used in PCR amplifications is Taq DNA polymerase that was isolated from thermophilic eubacterium Thermus aquaticus strain YT-1 (Taq) (6, 7). Currently it is isolated from recombinant E.coli overproducing Taq DNA polymerase

Unit Definition: One Unit of Taq DNA polymerase is defined as the amount of enzyme that will incorporate10 nM of dNTP into acid-insoluble material in 30 min at 75oC.

Concentration: The enzyme is supplied at a concentration of 5 Units / ul.

Specification: The enzyme is supplied at a concentration of 5 units/µL. The higher concentrated enzyme is supplied at 15-units/ µL. With every vial of Taq DNA polymerase, vials of 10X Taq Buffer and extra 100 mM MgSO4 are also supplied.

10 X Reaction Buffer: Includes 100 mM KCl, 200 mM Tris-HCl (pH 8.2), 100 mM Ammonium sulphate, 20 mM Magnesium sulphate and 1 % Triton X - 100.

Storage: Store at -20 ºC

Quality Assurance: The purity of Taq DNA polymerase protein is confirmed by 8% SDS-PAGE. It is highly purified as observed after staining with coomassie blue and then destaining. It is also free from any contaminating endonucleases and exonucleases.

Applications: Extensively used for general and specialized PCR including diagnostics, PCR cloning in T-tailed vector, genomic, RAPD, AP-PCR, etc.

Information for purchase of Taq DNA polymerase from VARDA Biotech:

To order " Taq DNA Polymerase" from VARDA, which is available on brand name " VarTeq" Please (click) Ordering Form

To know the terms and Conditions for Taq DNA Polymerase (click) Ordering Terms & Conditions